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pljm1 bcli lentivirus transfer vector  (Addgene inc)


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    Structured Review

    Addgene inc pljm1 bcli lentivirus transfer vector
    Pljm1 Bcli Lentivirus Transfer Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 400 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pljm1+bcli+lentivirus+transfer+vector/pLJM1-EGFP+(Plasmid+%2319319)/pm38451083-403-11-22
    Average 96 stars, based on 400 article reviews
    pljm1 bcli lentivirus transfer vector - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Construct:

    Article Title: Histone H2A variant H2A.B is enriched in transcriptionally active HSV-1 lytic chromatin
    Article Snippet: To insert the 3Xflag-tag sequence into pmacroH2A-C1, the gBlock (3Xflag-tag) DNA sequence (IDT) , and stop codon, flanked by BlpI and SalI restriction digest sites, was ligated in frame with pmacroH2A-C1 at the BlpI and SalI restriction sites. .. The pLJM1-BclI lentivirus transfer vector was constructed by removing EGFP from pLJM1-EGFP (Addgene) by AgeI and EcoRI restriction digest, and then adding in frame complementary oligonucleotides BclI fwd and BclI rev (IDT) , which contain the BclI cleavage site and overhangs compatible with AgeI and EcoRI sites. .. Plasmids pEGFP-H2A, pEGFP-H2B, pEGFP-H2A.B, pEGFP-macroH2A, pH2A-3Xflag, pH2B-3Xflag, pH2A.B-3Xflag, pmacroH2A-3Xflag and pLJM1-BclI were grown in dam - /dcm - competent E.coli (NEB).

    Article Title: Histone H2A variant H2A.B is enriched in transcriptionally active and replicating HSV-1 lytic chromatin
    Article Snippet: To insert the 3Xflag-tag sequence into pmacroH2A-C1, the gBlock (3Xflag-tag) DNA sequence (IDT) , and stop codon, flanked by BlpI and SalI restriction digest sites, was ligated in the frame with pmacroH2A-C1 at the BlpI and SalI restriction sites. .. The pLJM1-BclI lentivirus transfer vector was constructed by removing EGFP from pLJM1-EGFP (Addgene) by AgeI and EcoRI restriction digest, and then adding in frame complementary oligonucleotides BclI fwd and BclI rev (IDT) , which contain the BclI cleavage site and overhangs compatible with AgeI and EcoRI sites. .. Plasmids pEGFP-H2A, pEGFP-H2B, pEGFP-H2A.B, pEGFP-macroH2A, pH2A-3Xflag, pH2B-3Xflag, pH2A.B-3Xflag, pmacroH2A-3Xflag, and pLJM1-BclI were grown in dam - /dcm - -competent E.coli (NEB).

    Article Title: Histone H2A variant H2A.B is enriched in transcriptionally active and replicating HSV-1 lytic chromatin.
    Article Snippet: To insert the 3Xflagtag sequence into pmacroH2A-C1, the gBlock (3Xflagtag) DNA sequence (IDT) (Table 9), and stop codon, flanked by BlpI and SalI restriction digest sites, was ligated in the frame with pmacroH2A-C1 at the BlpI and SalI restriction sites. .. Cloning of flag and EGFP-tagged histones into lentivirus transfer vectors The pLJM1-BclI lentivirus transfer vector was constructed by removing EGFP from pLJM1-EGFP (Addgene) by AgeI and EcoRI restriction digest, and then adding in frame complementary oligonucleotides BclI fwd and BclI rev (IDT) (Table 8), which contain the BclI cleavage site and overhangs compatible with AgeI and EcoRI sites. .. Plasmids pEGFP-H2A, pEGFP-H2B, pEGFP-H2A.B, pEGFP-macroH2A, pH2A3Xflag, pH2B3Xflag, pH2A.B3Xflag, pmacroH2A3Xflag, and pLJM1-BclI were grown in dam-/dcm--compe tent E.coli (NEB).

    Cloning:

    Article Title: Histone H2A variant H2A.B is enriched in transcriptionally active and replicating HSV-1 lytic chromatin.
    Article Snippet: To insert the 3Xflagtag sequence into pmacroH2A-C1, the gBlock (3Xflagtag) DNA sequence (IDT) (Table 9), and stop codon, flanked by BlpI and SalI restriction digest sites, was ligated in the frame with pmacroH2A-C1 at the BlpI and SalI restriction sites. .. Cloning of flag and EGFP-tagged histones into lentivirus transfer vectors The pLJM1-BclI lentivirus transfer vector was constructed by removing EGFP from pLJM1-EGFP (Addgene) by AgeI and EcoRI restriction digest, and then adding in frame complementary oligonucleotides BclI fwd and BclI rev (IDT) (Table 8), which contain the BclI cleavage site and overhangs compatible with AgeI and EcoRI sites. .. Plasmids pEGFP-H2A, pEGFP-H2B, pEGFP-H2A.B, pEGFP-macroH2A, pH2A3Xflag, pH2B3Xflag, pH2A.B3Xflag, pmacroH2A3Xflag, and pLJM1-BclI were grown in dam-/dcm--compe tent E.coli (NEB).



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    pljm1 bcli lentivirus transfer vector  (Addgene inc)
    96
    Addgene inc pljm1 bcli lentivirus transfer vector
    Pljm1 Bcli Lentivirus Transfer Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pljm1+bcli+lentivirus+transfer+vector/pLJM1-EGFP+(Plasmid+%2319319)/pm38451083-403-11-22
    Average 96 stars, based on 1 article reviews
    pljm1 bcli lentivirus transfer vector - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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